14/ Shout-out to Zhihao Lai who developed this awesome vector-building system as a SIDE PROJECT during his PhD! 🧪 #Cloning#CloningSystems#plasmids#StructuralBiology#protein#sciencebsky.app/profile/davi...
Have you always wanted to build multi-gene baculovirus expression vectors without the pain? We introduce MoClo Baculo: + No PCR + No primers + Swap tags/ORFs easily + No more sequencing gazillion intermediate plasmids + Bonus: yeast compatibility 🧵 www.biorxiv.org/content/10.1...
Recombinant macromolecular complexes are often produced by the baculovirus system, using multigene expression vectors. Yet, the construction of baculovirus-compatible multigene expression vectors is c...
📜 MultiGreen: A multiplexing architecture for GreenGate cloning 🧑🔬 Vincent J. Pennetti, Peter R. LaFayette, Wayne Allen Parrott 📔 PLOS ONE 🔗 dx.plos.org/10.1371/jour...#PlantScience#Cloning#PlantSynthBio#SyntheticBiology#CloningSystems#GreenGate#PlantGoldenGate#PlantMolecularBiology
Genetic modification of plants fundamentally relies upon customized vector designs. The ever-increasing complexity of transgenic constructs has led to increased adoption of modular cloning systems for...