11/ Fun fact #2: No sequencing of intermediate products! No PCR/primers are used --> low mutation rate --> intermediate plasmids ("Level 1") can be validated only by restriction digestion --> a whole plasmid sequencing can typically be done only on the final multi-gene plasmid.

12/ Fun fact #3: Intermediate plasmids (Level 1) can be used for protein expression, making MoClo Baculo useful even if you wish to quickly build vectors that express only a single protein. (Though, in such case, you'd like to sequence these plasmids).