Discovering arcane controversies within fields is always fun 🧪🧶🧬. Recently @lindorfflarsen.bsky.social brought one to my attention: do high thermostabilities of ancestrally reconstructed sequences tell us anything about *real* ancestral seqs, or are they artifacts of the reconstruction process? 🧵

Background: ancestral sequence reconstruction (ASR) is a procedure for determining the amino acid sequence of a now-extinct progenitor sequence from its descendants, the extant sequences that have been identified and sequenced www.sciencedirect.com/science/article/pii/S002192582200878X