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Justine Debelius
@jwoodelius.bsky.social
Looking for stories in words and data and occasionally poop. Human gut microbiomes and weirdness. The typos and opinions are my own and not that of my employer. She/Her. Current: JHU. Past: KI, UCSD, CU Boulder
125 followers145 following138 posts
Reposted by Justine Debelius
AMmiscelena.bsky.social

Red's Burritos and Taylor Farm salads were out of stock last time I was shopping, which is either because they'd pulled them because of this... orrrrrrr just my dumb luck. Check the list.

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JDjwoodelius.bsky.social

I have SO many issues with phyloseq as a base package but kinda love micro shades 2 level bar plots I did some reading but I may go back to numerical ecology.

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JDjwoodelius.bsky.social

Are they based on a certain 2014 paper thatā€™s been refuted twice by chance? Also, if you donā€™t rarely, how do you handle the effect on depth in richness? How do you set your minimum sequencing depth for analysis or do you just combine samples w/ 100 seqs & 100K seqs?

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JDjwoodelius.bsky.social

Thatā€™s definitely where I like stacked bar plots. That was how we discovered they werenā€™t popping for fecal samples

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JDjwoodelius.bsky.social

1000% agree with ā€œcanā€™t just plot phylaā€. Just not sold on krona plots

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JDjwoodelius.bsky.social

Thank you!

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JDjwoodelius.bsky.social

Thank you!

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Reposted by Justine Debelius
KMdnakendra.bsky.social

Here's what I do. I don't do stacked plots as initial look (i make krona plots for each sample). I think stacked bar plots are only useful when carefully chosen (can just plot phyla!). They are the abstract of your data, you make those when you are in the writing phase. github.com/krmaas/bioin...

bioinformatics/GENERICdata.exploration.Rmd at master Ā· krmaas/bioinformatics
bioinformatics/GENERICdata.exploration.Rmd at master Ā· krmaas/bioinformatics

random collection of scripts used to process sequences - krmaas/bioinformatics

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JDjwoodelius.bsky.social

Now my data and trash can look like a dizzying array of skittles!!!

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Profile banner
JD
Justine Debelius
@jwoodelius.bsky.social
Looking for stories in words and data and occasionally poop. Human gut microbiomes and weirdness. The typos and opinions are my own and not that of my employer. She/Her. Current: JHU. Past: KI, UCSD, CU Boulder
125 followers145 following138 posts