Genome-wide dual CRISPRi-seq! Knocking down every gene with every other gene in the pneumococcus, identifying > 4000 genetic interactions (GIs), most of which have never been reported. www.biorxiv.org/content/10.1...

Dual CRISPRi-Seq for genome-wide genetic interaction studies identifies key genes involved in the pneumococcal cell cycle

bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

Most genes are not essential in any given bacteria under any given condition. This makes it hard to assign function to genes. Uncovering genetic interactions such as synthetic lethal (negative) GIs is a great way to figure out what a gene does. Here comes dual CRISPRi-seq!