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Natalie Turner
@natalieturnerau.bsky.social
Postdoc @ Scripps Research - Yates lab | ExtracellularVesicles | Mass Spectrometry | DIA | Bioinformatics | R | DIA-NN. Multi-omics enthusiast. Here to talk science & escape X. 🇦🇺 🇺🇸 🇬🇧 .
169 followers196 following76 posts

So far I have precipitated larger bound proteins with ACN, leaving the small peptides and hydrophobic molecules in the S/N. I have SepPak C18 cartridges for SPE. Looking for the best combination of loading/washing/elution buffers and whether to fractionate with increasing conc of elution buffer?

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NT
Natalie Turner
@natalieturnerau.bsky.social
Postdoc @ Scripps Research - Yates lab | ExtracellularVesicles | Mass Spectrometry | DIA | Bioinformatics | R | DIA-NN. Multi-omics enthusiast. Here to talk science & escape X. 🇦🇺 🇺🇸 🇬🇧 .
169 followers196 following76 posts