After a conversation with Bruker application specialist, the consensus looks like this: Bruker PepSep columns thread. @htlau.bsky.social

PepSep ULTRA: A column for most applications, you go with PepSep ULTRA (25cm, 75um ID, 1.5um beads). This will be good for low sample amounts as well as complex samples. Good for longer gradients <-> not recommended for <30min runs. So it will NOT be a column for very high throughput.