I made more tests with DIA from 100 pg to 150 ng, with gradients from 10 min to 90 min. Anything below 5 ng suffers from gradients above around 20 min. With higher inputs , longer gradients actually give you a tiny little bit more precursors. Full report incoming after I'm back from holidays :)

Guess that speaks to the sensitivity of the instrument..that long gradients actually dilute the signal for such low conc?