Are you advocating the general use of DDM? The public data I have seen wrt to DDM always focuses on its use to prevent protein/peptide loss in very small samples.

Believe it or not, it's the first time I've used it. And the increase in the identified peptides and proteins is independent of the input, from 10 ng down to 32 pg. So I was thinking to just always use it, but... Does it influence the mass spec in the long term in any way? Columns?