I was wondering the same thing-if morephus doesn't do it, I wonder if MetaMorpheus would. Hmmmm....

Ehhhhhhh....yes and no. I've tried.

Phenomenal! Thank you. I'll see how it goes- fingers crossed!!!

Thanks guys for the fast replies! I appreciate you all!

Yup. DDA, purified protein. I'll give this a shot.

Yah, used this. Staying away from $$. I should have probably mentioned freeware.

True true. Actually much better idea for some of things we are doing. But let's say we've already ran the DDA.

#massspecsos Best way/software for peptide mapping from limited proteolysis or full proteolysis experiments for a single, purified recombinant protein? Something that I can use/search against the Fasta for so the search would be our protein and contaminants with Thermo data.

Maybe next weekend. At the finish point, I was just excited to see flames where there was supposed to be and the only smoke I smelled was coming from the pellets.

Just finished replacing the hot rod and the fan (might as well since I had everything torn up). 4 hrs, 3 YouTube videos and enough cursing to make a sailor blush and we are back in business.