Microbiome twitter, we are looking for negative control samples from as many sources and kits as possible. If you've included blanks in your 16S or ITS amplicon seq project and the data is publicly available, drop a link in the thread! RTs appreciated!

The first and last extraction of every kit was a blank in the limony dataset. (These are released on SRA but not part of the r data package) www.biorxiv.org/content/10.1...

Lake iTag measurements over nineteen years, introducing the limony dataset

bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

We have several extraction blanks sequenced and in NCBI. I’ll look for links

Neg controls from extractions right? Mine aren't public but I'd be open to talking about your plans and sharing. Mars at uconn.edu

Pretty sure we did ITS and 16s blanks from the moBio HT kits: journals.asm.org/doi/10.1128/...

The BioSamples with NEG in the name: www.ncbi.nlm.nih.gov/bioproject/?...peerj.com/articles/127...

Bioproject PRJNA521988 has 3 negative controls called "BLANK"; the publication is linked to the Bioproject. There is also 1 "BLANK" in PRJNA641080; paper in press at AEM. I also have several others for projects in the works that I will be depositing by the end of the year. What's your timing?

www.ncbi.nlm.nih.gov/sra?linkname... The last 3 samples, labeled "blank"

We routinely include NTC, extraction blanks, and mock communities in our sequencing plates, e.g.: soil.copernicus.org/articles/9/2...soil.copernicus.org/articles/8/1...bg.copernicus.org/articles/18/...bg.copernicus.org/articles/16/...