Corresponding authors: @ehauptfeld.bsky.social@bedutilh.bsky.social
IntegRATe your taxonomic profiling by downloading RAT ⏬ from github.com/MGXlab/CAT_p.... RAT is run with CAT_pack reads 🐀🐈⬛🦇. (5/5)
CAT/BAT/RAT: tools for taxonomic classification of contigs and metagenome-assembled genomes (MAGs) and for taxonomic profiling of metagenomes - MGXlab/CAT_pack
Our study shows that by using this approach, RAT removes almost all falsely detected organisms in simulated data, and reduces the number of detected organisms in real metagenomic datasets by 80%! (4/5)
RAT largely removes this issue by rigorously prioritizing taxonomic signals by annotation reliability: Read maps to a MAG? -> assign to the same organism as the MAG. Read maps to an unbinned contig? -> same procedure. Only unmapped reads are annotated directly. (3/5)
Did you ever wonder why you can find Tree Lizard 🦎 DNA at the bottom of the ocean? Read-based shotgun metagenomics profiles notoriously include random species that have no business being in a sample. Reads are often short can therefore falsely match surprising organisms. (2/5)
Also see this nice summary on the Utrecht University website!