How does the ONT R.10.4.1 yield compare with R.9.4.1. I remember seeing some data from ages ago that showed that R.10 yield was lower. Still true?

I can’t really state that the yield for the R10.4.1 Flowcells is lower in comparison to the R9.4.1 cells.

No big difference lately. Had a run of 17gb

Not sure about lower yield, but we've definitely seen the N50 get much smaller using R.10s .

I think yields can be lower in a lot of hands, and many people (including us) hasn't quite adapted to figuring out how to modify extractions and preps to optimize yield on R10. Officially, I don't think ONT has stated that there should be an decrease in yield?

Not so it matters I think. We are getting fully closed chromosomes and plasmids running 16 E. coli on a flow cell and ONT only.