Ever wanted to edit a protein sequence inside a living mammalian cell? Maybe to site specifically incorporate a non-canonical residue? Perhaps you’d like to do a pull down without an antibody? Or use a small molecule fluorophore to image an endogenous protein? 1/4

Super Cool!

So did we! As part of our continued collaboration with the @Ophir_Shalem lab, we have developed an approach for intracellular protein editing by combining split inteins, endogenous tagging and genetic code expansion. 2/4