Sadly..

Looks like this is turning out to be a case in point. The engagement has dropped drastically here.. As a matter of fact, it was a discussion on proteoforms that had quite a lot of engagement from #TeamMassSpec in the other world last week..

Guess it's time for reverse migration.. Engaging #TeamMassSpec discussions happen more often there..

Dear team mass spectrometry, I am acquiring plasma proteomics data in DIA, 60SPD with Evosep-FAIMS (CV-45)Exploris 480, and the pq500 peptides spike in. Can you help me understand what kind of workflow and settings I should use on SP18 and/or on FragPipe-DIANN?

Staff Scientist 1 Mass Spectrometry-Based Proteomics of Alzheimer’s Disease - Bethesda, Maryland job with National Institute on Aging (NIA) | 11049 careers.cell.com/job... --- #proteomics #prot-jobs

Thanks.. Looking forward to connecting in twitter..

Thank you so much for the information.. It would be lovely to chat over DM.. I think we are not connected on twitter.. Could you please share your handle ?? Thanks..

#Astral#TeamMassSpec, what is an ideal SPD to be used if the aim is to quantify ~6k proteins per run?? The recent nDIA paper shows -9k proteins quantified (CV<10%) from 28-min active gradient runs.. That is about 50 SPD.. Has anybody tried 100 SPD or more?? Thanks..

Hello #TeamMassSpec#ComputationalMassSpectrometrist#Proteomics#MomentumBiowww.linkedin.com/jobs/view/37...

#TeamMassSpec.. a basic question.. If I have a peptide database (each entries with 8-15 amino acids long), and I am using it for spectral matching from a DDA data, would I be getting a reliable FDR validation since the decoy db will be crappy here??