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Bini Ramachandran
@itsbinir.bsky.social
Mass Spectrometry/Proteomics nerd....
145 followers117 following310 posts
Reposted by Bini Ramachandran
APangrist.bsky.social

Dear team mass spectrometry, I am acquiring plasma proteomics data in DIA, 60SPD with Evosep-FAIMS (CV-45)Exploris 480, and the pq500 peptides spike in. Can you help me understand what kind of workflow and settings I should use on SP18 and/or on FragPipe-DIANN?

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Reposted by Bini Ramachandran
Ppastelbio.bsky.social

Staff Scientist 1 Mass Spectrometry-Based Proteomics of Alzheimer’s Disease - Bethesda, Maryland job with National Institute on Aging (NIA) | 11049 careers.cell.com/job... --- #proteomics #prot-jobs

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BRitsbinir.bsky.social

#Astral#TeamMassSpec, what is an ideal SPD to be used if the aim is to quantify ~6k proteins per run?? The recent nDIA paper shows -9k proteins quantified (CV<10%) from 28-min active gradient runs.. That is about 50 SPD.. Has anybody tried 100 SPD or more?? Thanks..

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BRitsbinir.bsky.social

#TeamMassSpec.. a basic question.. If I have a peptide database (each entries with 8-15 amino acids long), and I am using it for spectral matching from a DDA data, would I be getting a reliable FDR validation since the decoy db will be crappy here??

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BRitsbinir.bsky.social

It's time to spill the beans and go into the details on my new nerdy mass spectrometrist role.. I will be officially moving to Boston to join Momentum Biotechnologies as the Director of Proteomics.. And my focus will be on, guess what, #chemoproteomics.. (1/6)

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Reposted by Bini Ramachandran
LKlkpino.bsky.social

Sunday evening at USHUPO will be a FeMS event! Specifically, the FeMS career transitions panel.

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BR
Bini Ramachandran
@itsbinir.bsky.social
Mass Spectrometry/Proteomics nerd....
145 followers117 following310 posts