Höstterminen av “Fika efter en forskare” är snart här! 🍂☕️ Vi är glada att kunna presentera tre tillfällen där tre fantastiska #forskare#medicinska#forskningumu.se/fika#forskomm#scicomm#forskning
You can now read more about our findings 👇 in a press release: umu.se/en/news/degrad…a umu.se/nyheter/nedbry #AR #bactera #scicom #sciencene #microbioe #womeninSTM #forskomm
I want to thank my coauthors Wei-Sheng Sun (on the left👇), @BerntssonLab (on my right), and our collaborators @TorrensGab83562 and @FelipecavaM from @CavaLab_MIMS All of us from @umeauniversity @Ucmr_sweden Picture: Anna Shevtsova 🧵3/3
We found that the enzyme works differently than predicted and is heavily regulated. We also got insight in how it is targeted to the #AMR#T4SSjournals.asm.org/doi/10.1128/... 🧵2/3
Antibiotic resistance is a large threat to human health and is getting more prevalent. One of the major contributors to the spread of antibiotic resistance among different bacteria is type 4 secretion systems (T4SS). However, mainly T4SSs from Gram-...
Today, mBio published our work on an enzyme that plays a crucial role in the transfer of #antibiotics#bacteria#infections#AMR transport. 🧵1/3
No words to describe my anger 'Russian activist and Putin critic Alexei Navalny dies in prison' www.theguardian.com/world/2024/f...
Opposition leader, 47, was being held in jail about 40 miles north of Arctic Circle
Further, AlphaFold2 modelling suggested that PrgK interacts with PrgL, another T4SS integral membrane protein. Using SEC-MALS we could show that this interaction was real.
The other two domains, LytM and CHAP, are instead involved in regulating the MUR domain. This results in a self-regulation of PrgK, which is likely very important to prevent cell toxicity. Interestingly, this self-regulation is lost when acting on cell walls from Gram-negatives.
PrgK has three extracellular domains. We here show that only one of the three domains is active, and in contrast to other known homologs in T4SSs it is actually a muramidase (MUR), instead of the proposed soluble lytic transglycosylase (SLT).
Check out our new preprint on PrgK, the cell-wall degrading enzyme of a Gram-positive Type 4 Secretion System that transports #antibiotic#virulence#AMR@rberntsson.bsky.socialwww.biorxiv.org/content/10.1...
