Universally SDS performs good> additional lysis of pellet and sup+pellet lysate is more comprehensive> Urea has bias to nuclear proteins...and Fig 4 is cool. Although, it is a bit alarming to see the amount of proteins being left out in the pellet.. dual lysis is the way to go?

BSbiorxiv-sysbio.bsky.socialFeb 22, 2024 1:08am

Lysis buffer selection guidance for mass spectrometry-based global proteomics including studies on the intersection of signal transduction and metabolism https://www.biorxiv.org/content/10.1101/2024.02.19.580971v1

Lysis buffer selection guidance for mass spectrometry-based global proteomics including studies on the intersection of signal transduction and metabolism https://www.biorxiv.org/content/10.1101/2024.02.19.580971v1

Prerequisite for a successful proteomics experiment is a high-quality lysis of the sample of interes