When you think it's over, it's not. There is still a lot of heat to dissipate in the golf of Mexico. www.nhc.noaa.gov/gtwo.php?bas... Orange is not a track, it is the development area.
NMR properties are an important part of the spectroscopic toolbox of ORCA 6 (@faccts-orca.bsky.socialdoi.org/10.1021/acs....#ORCAqc#ORCA6#NMR#ChemSky#CompChem
(2S,3S)-4-Fluorovaline (FVal) is an analogue of valine, where a single CH3 group is substituted by a CH2F group. In the absence of valine, E. coli valyl-tRNA synthetase uses FVal as a substitute, enabling the production of proteins uniformly labeled with FVal. Here, we describe the production and analysis of E. coli peptidyl–prolyl isomerase B where all 16 valine residues have been replaced by FVal synthesized with a 13C-labeled CH2F group. Although the melting temperature is lower by about 11 °C relative to the wild-type protein, the three-dimensional protein structure is almost completely conserved, as shown by X-ray crystallography. The CH2F groups invariably populate staggered rotamers. Most CH2F groups populate two different rotamers. The increased space requirement of fluorine versus hydrogen does not prohibit rotamers that position fluorine next to a backbone carbonyl carbon. 19F NMR spectra show a signal dispersion over 25 ppm. The most high-field shifted 19F resonances correlate with large 3JHF coupling constants, confirming the impact of the γ-gauche effect on the signal dispersion. The present work is the second experimental verification of the effect and extends its validity to fluorovaline. The abundance of valine in proteins and structural conservation with FVal renders this valine analogue attractive for probing proteins by 19F NMR spectroscopy.
Preprint, Maximizing NMR Sensitivity: A Guide to Receiver Gain Adjustment, Josh P. Peters, Jan-Bernd Hövener, Andrey N. Pravdivtsev arxiv.org/abs/2409.16411#NMRchat#NMR 🧲
Nuclear magnetic resonance is revolutionizing in parallel with the development of methodology and all electronics and hardware around it. The primary objective of such modifications is to make the...
No fun ahead...
A hurricane, which will be named Helene, is expected to form and rapidly intensify this week in the Gulf of Mexico before hitting Florida on Thursday or early Friday. Over the weekend, risks increased of a major hurricane landfall in the state.
In addition to inhabitants of the Big Bend and Panhandle areas, residents from Fort Myers, Fla., to Gulfport and Biloxi, Miss., should remain on alert
I had a great visit to the MagLab in Tallahassee. I saw a lot of weird magnet designs and new probes. I hope I can stay longer next time-lots of neat experiments to do.
📢 1.90 is rolling out now (10/11) We’re publishing a series of blog posts on Trust & Safety efforts at Bluesky. The first in the series covers the features we’re releasing in this version. Read it here: bsky.social/about/blog/0...
Trust and Safety (T&S) affects everything — from community policy and spam detection, all the way to the order that replies show up on a post. At Bluesky, the product team works hand-in-hand with T&S ...
The Third Element is now online! PCS Match: Assignment of PCS shifted spectra from a diamagnetic reference. Contact us for access: nmronline.uk#CompChem#ChemSky#NMR#NMRchat#NMROnline#SMASH2024 🧪🧬🧶
Thank you to all who have requested access to ELEMENTS, our FREE web NMR spectroscopy software. DNN autoCEST and ChemEx CPMG are now available! Contact us for access: nmronline.uk#CompChem#ChemSky#NMR#NMRchat#NMROnline#SMASH2024 🧪