Hey # TeamMassSpec, Have you ever considered DIA for small pulldown studies? I know that should definitely be one of the TMT sweetspots, but have not seen any back-to-back comparison so far..

We routinely use DIA to analyze pull-downs. I try to avoid TMT most of the time, but regardless I think our DIA results for pulldowns have been great.

TMT for pulldowns?? Used to avoid TMT for pulldowns because of the inherent variations between the test and controls..