Inerestingly, this picture changes when using fulltext search where algorithms based on Sequest (as implemented in ProteomeDiscover) dominate...

I think there are DIA applications were MS1-based quan has shown benefits (i.e. plexDIA). Also more recent algorithms like QuantUMS make use of this Information to inprove accuracy/precision…

.. but forgot that one needs it for MS1 full scan capability

Orbitrap-free Astral?

What do you measure and how?

At same time have 2 timsTOFs running for a year w/o performance drop. Have only cleaned ion transfer interface once which takes 5 minutes..

Quad cleaning every 3 months on multiple Exploris and Eclipse systems running 24/7. Individual systems seem generally more affected by charging than others requiring extra cleaning. Note that we have removed FAIMS devices. Possibly would keep dirt out but have no capacity for weekly cleaning…

Registration still open?

What ion current do you apply? Solvent details? Flow?

That said we still use original beads as I am too lazy to update the protocol, but use 96-well PVDF filter plates for bead retention instead of magnets. 4x96 samples prepped in one go in ~1.5h hands on time. Works robust for TMT or DIA or whatever: experiments.springernature.com/articles/10....