Trying to estimate popularity of search engines. (Something widespread I missed out?) Based on citations/year of initial publication it seems as MSFragger would take over soon...

Hey #TeamMassSpec#OrbiTOF Astral in their lab? Would be interested in comparisons. Where do both have their respective sweet spots?

Anyone having a good explanation why all Orbi systems require frequent cleaning while timsTOFs just continue to run stable ?

Has anyone tried already cleaning-up proteins from Trizol samples using SP2/SP3/SP4 ? Acetone precipitation does not work robustly..

Hey # TeamMassSpec, Have you ever considered DIA for small pulldown studies? I know that should definitely be one of the TMT sweetspots, but have not seen any back-to-back comparison so far..

Hi #TeamMassSpec#timsTOF-users Has anyone so far attempted to automatically trigger a first pass DIA-NN analysis of diaPASEF files directly from the HyStar queue?

Hi #TeamMassSpec, Does anyone have a simple script at hand to filter fasta files for a list genes or uniprot accessions?

FDR, as currently implemented, is probably broken. Short gradients, DIA, chimeric spectra, PTMs, emphasis on quant. Everyone talks about "how" to do FDR "right" but maybe we need to ask WHY we're doing it and if we should rethink the entire process based on the purpose.