RNA tertiary structure and conformational dynamics revealed by BASH MaP www.biorxiv.org/content/10.1101/2024.04.11.589009v1

RNA tertiary structure and conformational dynamics revealed by BASH MaP https://www.biorxiv.org/content/10.1101/2024.04.11.589009v1

The functional effects of an RNA can arise from complex three-dimensional folds known as tertiary st

Grateful to all co-authors! Special thanks to Drs. Dutta and Leone for initiating this #collaboration. This project has been an incredible journey of discovery, allowing me to learn and grow. Big thanks to the NCI K00 for their support!

Taken together, these results demonstrate that hepatocytes can synthesize DNA via endoreduplication, enabling liver regeneration even without two ORC subunits. #ORC#Endoreduplication#LiverRegeneration

Partial #hepatectomy#regenerating livers of dKO mice.

dKO female mice showed a significant decrease in size. We have also observed ~50% lethality of dKO female mice within the first month of life, but the remaining 50% survive beyond 4 months of age. #Female#morbidity#mortality than male mice.

The double knockout (dKO) of Orc1 and Orc2 led to another round of intriguing findings. H&E staining showed that dKO female livers exhibited significantly fewer but larger cells with notably larger nuclei compared to male livers. #SexSpecificDifferences

#EdU#Cre recombinase activity) were proficient in synthesizing DNA and incorporating EdU during liver regeneration in vivo.

Histological examination of the livers unveiled that Orc2 deletion correlated with the presence of fewer, yet larger nuclei and cells. Our flow cytometry analysis further substantiated that Orc2 deletion promotes endo-reduplication in #hepatocytes.

By employing #liver specific Alb-Cre, we found that body weights of the Alb-Orc2f/f mice were smaller than in the Orc2f/f animals of both sexes, though the liver size and liver size normalized to body weight was significantly smaller only in the females.

Through the breeding of Orc2f/f mice with Sox2-Cre mice, our study demonstrated the essential role of ORC2 in #embryonic#development. Notably, the proliferation rate of Orc2f/f mouse embryonic fibroblasts (MEF) infected with adeno-Cre was significantly impaired.